Data Acquisition and Imaging

If you need to book instrument use, training, or consulation please email: Mbcf@etsu.edu

Your core has many options available for data acquisition using our multimode plate reader or digital darkroom for collection of absorbance, fluorescent, or luminescent signal.

• Modulus Multimode Plate Reader

  User-friendly microplate reader with colorimetric,  fluorescent, and luminescent capabilities. Touch screen operation with data download to a USB drive. Accepts all 96-well plates conforming to the SBS plate standard.

  

  Absorbance Module

  The Absorbance module uses a large area photodiode detector to provide highly sensitive measurements covering a wide dynamic range with an accuracy that deviates less than 2%. The channel consists of a white LED with a spectral range of 450-750 nm.

  • Flexible Filter System
  • 
    0-5.0 OD Reading Range
  • 
    Single or Dual Wavelengths

  Absorbance Mode Filter Applications

  Wavelenth	Typical Applications
  450	ELISA, QuantiCleave Protease Assay
  560	BCA Protein Assays
  600	Bradford and Coomassie Blue Protein Assays, PeroXOquant Quantitative Peroxide Assay
  750	Lowry Protein Assay

  Fluorescence Module

  The Fluorescence module uses powerful wave-length matched, light-emitting PIN photodiodes (LEDs) as excitation sources. The narrow excitation/emission spectrum increases sensitivity and reduces non-specific light leakage. The fluorescence capability has a maximum linear dynamic range of 6 logs and a detection limit of 0.5 fmol/200 l or 1 ppt of fluorescein in a 96-well plate, or 30 pg/well dsDNA with a DNA quantification dye.

  • Epifluorescent Detection
  • 
    Multiple Optical Kits Available
  • 
    High Sensitivity

  Fluorescence Mode Optical Kit Applications

  UV Optical Kit Ex: 365 nm Em:410-460 nm	DNA Quantification Enzyme Activity (4-methyl-umbelliferone)
  Blue Optical Kit Ex: 490 nm Em: 510-570 nm	DNA, RNA, and Protein Quantification Protein Labeling (Florescein) Gene Expression (EGFP, rAcGFP)
  Green Optical Kit Ex: 525 nm Em: 580-640 nm	Nucleotide Labeling Protein Labeling (Rhodamine, Cy3) Enzyme Activity (Rhodamine)
  Red Optical Kit Ex: 625 nm Em:660-720 nm	Nucleotide or Protein Labeling (Cy5) RNA Quantification
  AFC Optical Kit Ex: 405 nm Em: 495-505 nm	Cell Viability Assays Cytotoxicity Assays Apoptosis Multiplex Assays

  Luminescence Module 

  The Luminescence module is separated from other measurement technologies and positioned directly above the sample well, maximizing light capture for increased sensitivity, with a detection limit of 3 x 10-21 moles Luciferase Detection or 1 x 10-18 moles ATP. The head-on photomultiplier tube has a spectral range of 350-650 nm and automatically adjusts for the optimum reading of bright or dim samples.

  Dual auto injectors are available for flash-based luminescence applications or dual-reporter assays. Each injector system has a volume range of 25 - 200 ul in 5 ul increments.

  • 8 Logs Dynamic Range
  • 
    Dual-masking System Reduces Crosstalk
  • 
    Very high sensitivity
• Syngene G:Box XX6

  The SYNGENE G:BOX iChemi XT is a high performing system suitable for fluorescence and chemiluminescence using a 6 megapixel camera.  It has a large aperture lens making it perfect for low light level applications where longer exposures are needed.